Chilling Tolerance
Objective: Identify the mechanistic basis of chilling tolerance in sorghum to accelerate marker-assisted breeding
Objective: Identify the mechanistic basis of chilling tolerance in sorghum to accelerate marker-assisted breeding
Project Description: Susceptibility to chilling temperatures is a major limiting factor of sorghum production in Kansas. Our research focuses on identifying the mechanistic basis of cold tolerance using multi-tier approaches: QTL mapping, transcriptomics and lipidomics. The outcome of our research can aid in developing molecular markers for marker-assisted selection.
Project Description: Susceptibility to chilling temperatures is a major limiting factor of sorghum production in Kansas. Our research focuses on identifying the mechanistic basis of cold tolerance using multi-tier approaches: QTL mapping, transcriptomics and lipidomics. The outcome of our research can aid in developing molecular markers for marker-assisted selection.
Experiments:
Experiments:
- Transcriptome Analysis
Evaluate transcriptome changes induced by chilling stress in BTx623, a chilling susceptible parent line, and Nui Sheng Zui (NSZ), a chilling tolerant Chinese landrace. Transcriptomic changes were evaluated 36 h after being subjected to chilling stress using transcriptomic pipeline
DNA Subway
RNA-Seq data analysis using Linux
- Lipidomics
- Metabolite Profiling (Dhurrin and Sugars)
- GWAS
- 2016 Field Season
Results:
Results:
- UAV imagery from Asebedo lab: smb://catfiles.users.campus/workarea$/agr/shared2/precision-ag